Delay of PV-induced iκbα mRNA synthesis by TSA addition 1 HISTONE DEACETYLASE INHIBITOR TRICHOSTATIN A SUSTAINS SODIUM PERVANADATE-INDUCED NF-κB ACTIVATION BY DELAYING IκΒα mRNA RESYNTHESIS - COMPARISON WITH TNFα
نویسندگان
چکیده
Abbreviations: ALLN, N-acetylleucylleucylnorleucinal; ChIP, chromatin immunoprecipitation; EMSA, electrophoretic mobility shift assay; ERK1/2, extracellular signal-regulated kinase 1/2; HAT, histone acetyltransferase; HDAC, histone deacetylase; HIV LTR, HIV long terminal repeat; H2O2, hydrogen peroxide; IKK, IκB kinase; MAPK, mitogen-activated protein kinase; MSK1, mitogenand stress-activated protein kinase-1; NF-κB, nuclear factor kappa B; NT, non treated; P-, phosphorylated residue; PMA, phorbol myristate acetate; PTPase, protein tyrosine phosphatase; PV, sodium pervanadate; qRT-PCR, quantitative real-time reverse transcriptase-PCR; RNA Pol II, RNA polymerase II; RPA, ribonuclease protection assay; SAHA, suberoylanilide hydroxamic acid; TNFα, tumor necrosis factor alpha; TSA, trichostatin A.
منابع مشابه
O-GlcNAc transferase inhibits LPS-mediated expression of inducible nitric oxide synthase through an increased interaction with mSin3A in RAW264.7 cells.
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT), which catalyzes the addition of a single β-N-GlcNAc unit to target proteins, has been shown to act as a transcriptional regulator. In the current study, we discovered that OGT exerted inhibitory effects on the LPS-driven activation of NF-κB and inducible nitric oxide synthase (iNOS). In response to LPS, OGT exhibited an increased intera...
متن کاملTwo histone deacetylase inhibitors, trichostatin A and sodium butyrate, suppress differentiation into osteoclasts but not into macrophages.
Histone deacetylase (HDAC) inhibitors are emerging as a new class of anticancer therapeutic agents and have been demonstrated to induce differentiation in some myeloid leukemia cell lines. In this study, we show that HDAC inhibitors have a novel action on osteoclast differentiation. The effect of 2 HDAC inhibitors, trichostatin A (TSA) and sodium butyrate (NaB), on osteoclastogenesis was invest...
متن کاملEnhancement of Inflammatory Protein Expression and Nuclear Factor Κb (NF-Κb) Activity by Trichostatin A (TSA) in OP9 Preadipocytes
The production of inflammatory proteins such as interleukin-6 (IL-6) by preadipocytes and mature adipocytes is closely associated with the impairment of systemic glucose homeostasis. However, precisely how the production is regulated and the roles of histone deacetylases (HDACs) remain largely unknown. The aim of this study was to establish whether HDAC inhibitors affect the expression of infla...
متن کاملHistone deacetylase inhibitors suppress rheumatoid arthritis fibroblast-like synoviocyte and macrophage IL-6 production by accelerating mRNA decay
BACKGROUND Histone deacetylase inhibitors (HDACi) display potent therapeutic efficacy in animal models of arthritis and suppress inflammatory cytokine production in rheumatoid arthritis (RA) synovial macrophages and tissue. OBJECTIVES To determine the molecular mechanisms contributing to the suppressive effects of HDACi on RA synovial cell activation, using interleukin 6 (IL-6) regulation as ...
متن کاملRedirection of Epithelial Immune Responses by Short-Chain Fatty Acids through Inhibition of Histone Deacetylases
Short-chain fatty acids (SCFAs) are products of microbial fermentation that are important for intestinal epithelial health. Here, we describe that SCFAs have rapid and reversible effects on toll-like receptor (TLR) responses in epithelial cells. Incubation of HEK293 or HeLa epithelial cells with the SCFAs butyrate or propionate at physiological concentrations enhanced NF-κB activation induced b...
متن کامل